Because of the exhaustion of high-grade ore due to large-scale exploitation, the worlds demand for metals has gradually increased enormously as a result of industrialization. As a result, our challenge is to process lean grade ores in order to meet the needs of the majority of industries. Bioprocessing methods have attention-grabbing complexity in handling ores. Furthermore, as for the conventional inorganic reagents used in this work, biologically derived products are nontoxic and ecologically benign. The use of novel methods like bio-leaching, bio-flotation, and bio-flocculation for the beneficiation of complex ores has become a reality. We prepared 9 K media culture for the growth of Thiobacillus ferrooxidans as a microorganism and the use of the microbe as a substitute for NaCN as a depressant of the sphalerite mineral. The Zeta Potential studies were carried out in order to learn about the surface properties of minerals. Finally, the mineral recovery percentage was increased by using different microorganisms for galena and sphalerite ore minerals.