This study explores the potential of Bacillus subtilis and calcium lactate in enhancing the compressive strength and self-healing properties of concrete. Bacillus subtilis, a spore-forming bacterium, is known for its ability to produce antibiotics, which can aid in the survival of the microbe and eliminate rival microorganisms. Whereas calcium lactate, with its white powder appearance and efflorescent odor, serves as a source of calcium for the precipitation of calcium carbonate, a key component in concrete healing. Two cases were considered, one without the addition of calcium lactate and bacteria (jelly form), and the other with their incorporation. Specimens were prepared accordingly and were subjected to compressive strength testing at 7, 14, and 28 days. The self-healing ability of the specimens was observed 12 months later. Results indicate that the inclusion of calcium lactate and Bacillus subtilis in concrete mixtures shows promise for enhancing compressive strength, however, lesser performance in self-healing capabilities.