Scholarlink

Synovial joint fluid is the lubricant and nutrient of articular joint cartilage. Osteoarthritis research recently focused on genetics, epigenetics, proteomics, metabolomics and lipidomics. This technology not only allows the identification of phenotypes and endotypes but may also monitor the clinical, molecular and therapeutic stages of the disease. In this review, we summarized biomarker-based diagnostics of osteoarthritis. Our focus was the synovial joint fluid. Affinity chromatographic and omic-based strategies are presented. The limitations were highlighted. Insight into synovial joint fluid processing is provided. IMAC sorbent synthesis is discussed. LC–MS/MS capability after phosphopeptide enrichment is described. Outcomes of our pilot study are presented. The isolation and detection capabilities of the IMAC sorbent for other phosphostructures and their clinical use will be examined in future.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Synovial Joint Fluid Omics Researches in Osteoarthritis: An Affinity Chromatography-Based Perspective

  • 章节

  • 发表日期：

    2025年

查看全文
  • Serhat Aladağ,
  • Ilayda Demirdiş,
  • Burcu Gökçal Kapucu,
  • Bilge Başak Fidan,
  • Batuhan Erhan Aktaş,
  • Merve Gizer,
  • Ozan Kaplan,
  • Idil Yet,
  • Melis Şardan Ekiz,
  • Petek Korkusuz,
  • Mustafa Çelebier,
  • Ömür Çelikbıçak,
  • Ali Tuncel,
  • Feza Korkusuz

摘要

Synovial joint fluid is the lubricant and nutrient of articular joint cartilage. Osteoarthritis research recently focused on genetics, epigenetics, proteomics, metabolomics and lipidomics. This technology not only allows the identification of phenotypes and endotypes but may also monitor the clinical, molecular and therapeutic stages of the disease. In this review, we summarized biomarker-based diagnostics of osteoarthritis. Our focus was the synovial joint fluid. Affinity chromatographic and omic-based strategies are presented. The limitations were highlighted. Insight into synovial joint fluid processing is provided. IMAC sorbent synthesis is discussed. LC–MS/MS capability after phosphopeptide enrichment is described. Outcomes of our pilot study are presented. The isolation and detection capabilities of the IMAC sorbent for other phosphostructures and their clinical use will be examined in future.