It has been found that mushrooms contain phenolic compounds, aldehydes and ketones that render them antimicrobial properties. Its antimicrobial activity can be exploited to make antimicrobial plasters, which can kill bacteria on wound surfaces and block bacteria from entering wounds and causing infections. Mushroom extracts were made from Pleurotus Ostreatus, Lentinula Edodes, Agaricus Bisporus and brown and white Hypsizygus Tessulatus in ethanol with the ratio of 18 g: 50 ml and was evaporated and stored in 33% ethanol. Their antimicrobial activities were tested using the disk diffusion method, and we narrowed down the most effective mushroom. Next, we tested the different parts and dryness of the selected mushroom before incorporating the best extract into hydrogels. We further tested the hydrogels’ absorption, breathability and ability to kill and block bacteria. The ethyl acetate extract of fresh whole Brown Hypsizygus Tessulatus displayed the best antimicrobial properties, while Brown Hypsizygus Tessulatus showed the best results overall in both blocking and killing bacteria–fresh whole Brown Hypsizygus Tessulatus extract was able to block 100% of bacteria while dried cap Brown Hypsizygus Tessulatus extract killed 96.1% of bacteria. The hydrogel made with extracts was also able to absorb water and allowed oxygen to pass through.