The Retinal Pigment Epithelium (RPE) supports retinal health and enables the maintenance of basic retinal functions. However, it is susceptible to oxidative damage induced by Reactive Oxidative Species (ROS). This increases its liability to degenerative retinal diseases as damaged RPE cells cannot be replaced spontaneously. Dietary carotenoids, like lutein, can protect the RPE from oxidative damage. However, the specific effectiveness of lutein against H2O2, a primary ROS in the retina, have not been explored. Hence this experiment determines if lutein pre-absorption enhances the protection of RPE cells against H2O2 by measuring cell viability. ARPE-19 cells were thawed, cultured, and split into wells before being treated with differing concentrations of lutein (2 μM, 1 μM, 0.2 μM, 0.1 μM, 0 μM) and exposed to H2O2 before undergoing the CCK-8 cell viability assay. The assay shows that there is no statistically significant difference in the average cell viability between 0 μM and 0.1 μM, and 0 μM and 0.5 μM lutein respectively. This suggests that lutein at small concentrations between 0.1 μM and 0.5 μM respectively are inadequate to act as a shield against H2O2. A statistically significant difference in the average cell viability between lutein concentrations higher than 1 μM and 0 μM, showing they can efficiently guard RPE cells against H2O2. However, increments in lutein concentrations do not always entail an increased protective effect. This could be attributed to errors while preparing for the experimentation.