The word flavonoid comes from the Latin word “flavus,” which means yellow. This class of major polyphenolic compound is known for conferring colors, flavors, and bioactivities in flowers, fruits, and plants. The presence of bioflavonoids in traditional medicines has been popular for ages. However, in recent times, the use of flavonoids as bioactive nutraceuticals has gained particular prominence especially due to their impact in treating different lifestyle-based disorders and life-threatening diseases like cancers. Based on their unforeseen potential in medicine industry, researchers have tried to extract these phyto flavonoids for ages. Different traditional methods like maceration, percolation, boiling, refluxing, soaking, use of soxhlet apparatus, and hydro-distillation are few of the age-old techniques that have been extensively used for bioflavonoid extraction. But the excess consumption of organic solvents in these techniques, added with lack of precision in the extraction techniques, long extraction times, and lower selectivity rates, compromises the overall stability, purity, and overall yield of the final bioactive components. And thus these low quality minute amounts of bioactive flavonoids compromises the overall efficacy of the nutraceuticals. However, in recent years, with the inclusion of various advanced techniques like ultrasound-assisted extractions, supercritical fluid-based extractions, microwave-based extractions, etc., along with their combined techniques, have opened newer dimensions for flavonoids extraction from a plethora of natural matrices with utmost efficacy. Keeping these recent high-throughput techniques in mind, in this study, an attempt has been made to collate these different techniques for bioflavonoids extraction based on specificity of phyto flavonoids from different natural matrices.