Food products are frequently susceptible to deterioration during manufacturing, storage, shipping, demanding prompt, and effective quality testing. Gas sensors have recently become popular due to their ability to detect gases rapidly and sensitively, making them perfect for assessing food quality. Electrochemical gas sensors feature high selectivity, sensitivity, fast response, and a long lifespan. Through a chemical interaction with the target gas, they are meant to measure gas concentrations by producing an electrical signal that is proportionate to the gas concentration. Since ammonia (NH3) is a primary toxic gas that can be discovered in food products and poses a threat to human health, it is critical to monitor and identify it. Hence, in this work, Manganese dioxide (MnO2) nanoparticles were synthesized using a hydrothermal technique and proposed for the detection of ammonia in an alkaline medium. The electrode behavior was examined using cyclic voltammetry. Further, various characterization studies such as X-ray diffraction (XRD) and field Emission Scanning Electron microscopy (FESEM) were used to measure size and phase structure of the nanomaterials. The drop coating of ammonia (NH3) solution onto MnO2/GCE enabled the fabricated electrode to be utilized as an electrochemical sensor for NH3 detection in spoiled meat products. The Cyclic Voltametric (CV) behavior of NH3/MnO2/GCE was investigated in 1 M KCl and 0.05 M K3[Fe(CN)6]. This led to the formation of a manganese-ammonia complex and revealed the nobility of the fabricated electrode. The MnO2/GCE displayed high sensitivity for the detection of ammonia (NH3) in an alkaline medium and was found to be suitable for spoiled meat products.