This study explores the psychological effects of human-machine interaction, particularly focusing on the phenomenon of the uncanny valley within the context of virtual reality (VR). This study specifically investigates the relationship between oculomotor dynamics, EEG asymmetry, and perceived uncanniness in an immersive setting with a realistic human avatar that can generate uncanny feelings. Participants engaged in a VR scenario designed to optimize their sexual interest. Eye-tracking and EEG data were collected during these interactions to assess the predictors of uncanniness. The final regression model identified significant predictors, including EEG asymmetry in frontal and parietal regions and nonlinear indices of gaze behavior. The findings suggest that the more a participants’ attention was attracted towards specific cues, the higher the uncanniness. The interplay between EEG asymmetries and gaze behavior furthermore indicates that these physiological and behavioral responses are closely linked to the perception of uncanniness, offering insights into how humans interact with increasingly lifelike technologies. These insights contribute to a better understanding of HMI’s potential and may inform the development of more engaging and psychologically attuned virtual content.