In this research work, the single jersey fabric samples are prepared with 100% cotton, polyester/cotton, polyester-lycra, and cotton-lycra by varying yarn count and loop length. The influence of fabric parameters such as type of fiber, yarn count, loop length and fabric direction have been analyzed for seam strength and seam efficiency. The results revealed that in 100% cotton fabric, seam strength increases with a rise in the yarn count and falls in the loop length. Also the seam strength decreases when the seam is prepared in course wise direction (0°) as compared to wale wise (90°) and bias (45°) direction. It is concluded that for polyester/cotton fabric, medium range of loop length has the lowest seam strength value in the bias direction. It is noticed that a similar trend was observed for loop length and yarn count in polyester-lycra and cotton-lycra fabrics. It is noticed that for 100% cotton fabric, the seam efficiency increases with a rise in loop length, yarn count and fabric direction. For 100% cotton seam efficiency range from 64% to 98% and for polyester/cotton fabrics, the seam efficiency ranges from 80% to 100%. While in polyester-lycra and cotton-lycra the seam efficiency is observed to be between 60% and 100%. The ANOVA results showed that for seam strength, loop length has the highest contribution for 100% cotton and cotton-lycra fabric. For seam efficiency, yarn count and loop length combine has higher contribution for cotton fabrics. Hence, it can be concluded that seam strength, and efficiency can be improved by selecting suitable values of fabric particulates.