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The process of Microbial Induced Calcium Carbonate Precipitation (MICP) is an eco-friendly and promising technology for soil stabilization and construction. This study utilized Sporosarcina ureilytica, a non-pathogenic, gram-positive bacterium, to carry out MICP. The bacteria were sourced from Kien Giang province, Vietnam corals, and used in their spore state, demonstrating resilience to high alkalinity and urea concentrations. Nano calcite was employed as a nucleus for crystallization to expedite the MICP process. Almost all organic nutrient sources for biocementation were eliminated in this study. The results showed a significant increase in bulk density, with large soil samples exhibiting a 20% increase from their initial state, indicating improved material density, strength, and stability. Noteworthy changes in grain size distribution were observed, indicating enhanced soil compactness. Thermal analysis revealed a four-step degradation process, highlighting the presence of imperfect and hydrated CaCO2 in MICP-treated samples. Microstructure analysis using an optical microscope and SEM confirmed the impact of MICP on biocementation through grain bridging formation. Moreover, compressive strength and anti-erosion tests substantially improved the treated soil, while anti-corrosion tests indicated enhanced durability. These findings underscore the potential of MICP for sustainable construction and environmental protection, offering a reliable and cost-effective solution for soil stabilization.

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Increasing Bio-Cementation Efficiency Through Microbially Induced Calcium Carbonate Precipitation with Nano-Calcite for Sand, Sandy Soil, and Granular Materials

  • 会议论文

  • 发表日期：

    2025年

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  • Nguyen Ngoc Tri Huynh,
  • Nguyen Hoang Thien Khoi

摘要

The process of Microbial Induced Calcium Carbonate Precipitation (MICP) is an eco-friendly and promising technology for soil stabilization and construction. This study utilized Sporosarcina ureilytica, a non-pathogenic, gram-positive bacterium, to carry out MICP. The bacteria were sourced from Kien Giang province, Vietnam corals, and used in their spore state, demonstrating resilience to high alkalinity and urea concentrations. Nano calcite was employed as a nucleus for crystallization to expedite the MICP process. Almost all organic nutrient sources for biocementation were eliminated in this study. The results showed a significant increase in bulk density, with large soil samples exhibiting a 20% increase from their initial state, indicating improved material density, strength, and stability. Noteworthy changes in grain size distribution were observed, indicating enhanced soil compactness. Thermal analysis revealed a four-step degradation process, highlighting the presence of imperfect and hydrated CaCO2 in MICP-treated samples. Microstructure analysis using an optical microscope and SEM confirmed the impact of MICP on biocementation through grain bridging formation. Moreover, compressive strength and anti-erosion tests substantially improved the treated soil, while anti-corrosion tests indicated enhanced durability. These findings underscore the potential of MICP for sustainable construction and environmental protection, offering a reliable and cost-effective solution for soil stabilization.