Oil bearing evaluation is an important part of shale reservoir evaluation. Conventional experimental analysis techniques (such as rock pyrolysis S1, oil saturation, etc.) can only give the total oil content of shale, but cannot give the in-situ location of shale oil in micro and nano pores, which cannot describe the three-dimensional spatial distribution characteristics of shale oil. In this paper, the innovative method of closed pressure coring + liquid nitrogen freezing preservation and transportation + sample cutting and preparation under liquid nitrogen vapor environment + automatic cold table protection of crude oil light hydrocarbons was adopted to carry out laser confocal analysis during the whole process of freezing, to achieve accurate determination of total shale oil content, in-situ quantitative detection of shale oil quality, and quantitative characterization of shale oil quality in micro-regions. The current research shows that Gulong shale oil has six micro-distribution types, and the coexistence of “primary reservoir” and “micro-migration” of shale oil has been found. Micron-scale bedding fractures communicate the nano-pores in the matrix, forming a dual-pore media flow network of nano-pores and micron-cracks, which greatly reduces the starting pressure of shale oil. Most shale oil with a reservoir aperture of less than 50 nm can be produced.