Characterization Techniques for the Chitosan Nanoparticles and Derivatives
摘要
This chapter has provided a brief summary of various characterization techniques involved in the structural confirmation and to demonstrate the various properties of the chitosan derivatives and its nanoparticles. It has provided an overview of the primitive techniques such as UV–Visible spectroscopy and Fourier transform infra-red spectroscopy (FTIR). It has also demonstrated the spectra of some particular chitosan derivatives and nanoparticles. Apart from this information, it has also provided the details of the X-ray diffraction analysis, scanning electron microscopy (SEM), and transmission electron microscopy (TEM) which are important and fundamental techniques to demonstrate the crystallinity and morphology, respectively. The chapter has also provided the details of CS-TM-PEG copolymer-insulin nanoparticles by TEM. It has also provided the details of structural elucidation by nuclear magnetic resonance spectroscopy (NMR). It has also given details of Dynamic Light Scattering (DLS). The chapter has also highlighted the details of advanced techniques such as Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Characterization of Gd (TPyP) conjugated chitosan nanoparticles have also been presented. The importance of zeta potential measurements for the chitosan nanoparticles has been evaluated. Darkfield microscopy has been used for the characterization of chitosan-capped gold nanoparticles. It has also provided the details of an instrument that functions by considering an aerodynamic and optical property such as time-of-flight and light-scattering intensity for counting and size distribution profile of particles and is termed as Aerodynamic Particle Sizer (APS). Moreover, it has also given the details of Scanning Mobility Particle Sizer (SMPS) and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS). It has also summarized the details of size exclusion chromatography. All these techniques have been emerged as a powerful tool to comprehend the properties of the chitosan nanoparticles for various applications.