Voltammetry has emerged as a highly sensitive and selective technique for the detection of biomolecules in various fields, including healthcare, environmental monitoring, and food safety. Voltammetry-based biosensors utilize the electrochemical properties of molecules to detect specific analytes, offering a rapid, accurate, and cost-effective approach. These biosensors have been successfully applied to detect glucose, neurotransmitters, cancer biomarkers, toxins, and infectious disease biomarkers, among others. The advantages of voltammetry-based biosensors include high sensitivity, selectivity, and miniaturization potential, making them an attractive option for point-of-care diagnostics and real-time monitoring. This chapter highlights the significance of voltammetry in biosensing applications, paving the way for further research and development in this field.