Scholarlink

Polymerase chain reaction (PCR) is a molecular technique used to exponentially increase the amount of DNA for the purpose of testing and analysis. This method enables the rapid amplification of certain genes or DNA fragments in vitro. PCR technology replicates DNA in a manner similar to its natural process in living organisms and specifically amplifies a targeted section of DNA outside of a living organism. The PCR process is analogous to regular DNA replication and consists of three sequential phases. Initially, the template DNA undergoes denaturation, transitioning from a double-stranded configuration to a single-stranded configuration. Subsequently, the primer is introduced and joined with the template, facilitating the completion of the replication process. Finally, with the presence of DNA polymerase and substrate, complementary DNA is synthesized. The process of repeating these three heat reactions is referred to as a cycle, during which a significant quantity of DNA fragments situated between the two primer sequences can be amplified after 20–40 cycles.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Common Laboratory Testing Methods Used for Examination of Intraocular Fluid

  • 章节

  • 发表日期：

    2025年

查看全文
  • Yong Tao,
  • De-Kuang Hwang

摘要

Polymerase chain reaction (PCR) is a molecular technique used to exponentially increase the amount of DNA for the purpose of testing and analysis. This method enables the rapid amplification of certain genes or DNA fragments in vitro. PCR technology replicates DNA in a manner similar to its natural process in living organisms and specifically amplifies a targeted section of DNA outside of a living organism. The PCR process is analogous to regular DNA replication and consists of three sequential phases. Initially, the template DNA undergoes denaturation, transitioning from a double-stranded configuration to a single-stranded configuration. Subsequently, the primer is introduced and joined with the template, facilitating the completion of the replication process. Finally, with the presence of DNA polymerase and substrate, complementary DNA is synthesized. The process of repeating these three heat reactions is referred to as a cycle, during which a significant quantity of DNA fragments situated between the two primer sequences can be amplified after 20–40 cycles.