Breeding of crop plants has achieved notable success in developing cultivars that significantly boost agricultural production in favorable conditions. These cultivars, which are typically homozygous inbreds or hybrids descended from inbred parents, are produced under ideal conditions in the field and thrive especially in regions with sufficient moisture and nutrients. These ideal circumstances are, nevertheless, uncommon in the world at large, with a sizable portion of arable land being judged unsuitable for agricultural use. Agricultural marginal land is typically located in semi-arid or saline regions, has little soil depth, low production, and is prone to erosion. It is also anticipated that the effects of climate change and the ongoing depletion of the world’s soil and water resources would make these difficult circumstances much more severe. Crop wild relatives, or CWRs, are frequently bred for their ability to withstand biotic stress; typically, they possess traits that allow them to flourish in challenging conditions. Using CWRs directly for varietal development initiatives is often not advantageous because of their high frequencies of agronomically unfavorable genes and crossover or possible obstacles to chromosomal introgression with crops species. Linkage drag may cause unwanted traits in the progeny when phenotype-linked genomic regions, or quantitative trait loci (QTLs), are introgressed from wild germplasm. On the other hand, modern breeding practices focused on broad adaptation have led to decreased genetic diversity and heightened susceptibility to both biotic and abiotic stressors. There is a possibility to utilize genetic diversity instead of advocating for genetic consistency in breeding, aiming to improve the adaptability of crops for less favorable lands. In this chapter, we have detailed the adaptive traits that may increase crop variety productivity in challenging circumstances, and we offer recommendations on how to efficiently and rapidly utilize these wild relatives for prebreeding research projects that involve the introgression of foreign genes.