In this chapter, the author focuses on the remarkable continuous advancements that have been made regarding the utilization of the bio-compatible and stimuli-responsive capabilities of carbohydrate polymer-based hydrogels for biomedical applications in tissue engineering. Carbohydrate polymer-based hydrogels have found extensive utility in diverse fields, encompassing biomedicine, agriculture, food science, and environmental engineering. Hydrogels, intricate three-dimensional networks crosslinked to retain substantial water quantities, have garnered notable attention across scientific and industrial sectors owing to their distinctive characteristics. Carbohydrate polymers, derived from natural sources like cellulose, chitosan, starch, and alginate, have emerged as promising foundations for hydrogel construction. In biomedicine, these hydrogels exhibit significant potential in drug delivery systems, wound healing, and tissue engineering due to their biocompatibility and bioactivity. The authors discussed the many types of polymers that may be employed in the production of hydrogels, as well as the spectro-chemical analysis of these polymers and the following uses of hydrogels in the medical field. Researchers have spent a lot of time studying the biocompatibility, pH sensitivity, and temperature sensitivity of a wide range of polymers because these materials have different properties and uses.