Bioenzymes derived from organic sources exhibit remarkable potential in enhancing soil engineering properties, rendering them highly suitable for diverse applications within geotechnical engineering realms, including grouting and subgrade soil stabilization. Their efficacy has been notably demonstrated in pavement stabilization and sites predominantly subject to static loading conditions. However, this current research aims to delve into the validation of bioenzymes’ effectiveness specifically in soil stabilization scenarios where dynamic loading, particularly seismic or earthquake-induced forces, may be anticipated. A key concern is the existence of an underneath layer of soft clay under the soil layers, as seismic waves traveling through this layer can potentially amplify site-specific ground motions, leading to unexpected failures at the ground surface. While seismic waves typically dissipate their energy over distances due to damping effects and other factors, the proximity of soft clay layers can lead to amplification, exacerbating ground instability. This paper undertakes the evaluation of bioenzyme treatment efficacy under both static and dynamic loading conditions at sites prone to seismic activity. The research focuses on assessing the ability of bioenzyme to mitigate site amplification caused by soft clay layers during seismic wave propagation. The study aims to determine the suitability and performance of bioenzyme treatment in scenarios involving dynamic loading, thereby providing crucial insights into its effectiveness in stabilizing soil under seismic conditions.