The purpose of this experiment is to understand the gasification operation of waste biomass of wood chips or sawdust. The point of most concern is that biomass gasification will produce hydrogen-rich syngas, which can be used to generate electricity by a gas turbine generator or synthesized by catalysts to obtain methanol or other chemicals, which is an important energy or chemical source with considerable future development potential. Therefore, this study is to explore the basic properties of waste biomass and use the response surface methodology (RSM) to explore the operating parameters of gasification temperature (X1), residence time (X2) and the effect of different intake oxygen ratios (X3) on hydrogen production (Y1) and hydrogen to carbon monoxide ratio (Y2) in a gasifier, and then adding steam and catalysts into the process at the optimal parameters for comparison of hydrogen production. According to the experimental results, the gasification temperature is the most significant factor, and under the conditions of X1: 850 °C, X2: 20 min, X3: 0 vol%, there is the best hydrogen production (Y1) of 26.38 vol%, and the conditions of X1: 650 °C, X2: 20 min, X3: 0 vol% has the best hydrogen to carbon monoxide ratio (Y2) of 1.69. Under the optimal operating conditions, adding water vapor to the gasifier, the hydrogen production increased by 72.9%, and the hydrogen to carbon monoxide ratio increased by 79.0%. With the addition of Ni/SiO2 catalyst, the hydrogen production increased by 45.9%, and the hydrogen to carbon monoxide ratio increased by 27.1%.