Slope stability refers to the ability of a slope to resist mass movement or sliding. Seismic events can induce additional forces and stresses on slopes, making its stability even more challenging to assess. This study presents a stability analysis under seismic loading on a railway slope within the Barkheda–Budni 3rd Line Project situated in the western central railway (WCR) region of Bhopal division. A thorough site investigation program was conducted and soil samples from the following chainages: Chainage 9 + 11 KM, Chainage 10 + 580 KM, and Chainage 11 + 300 KM were collected and subjected to laboratory testing. GeoStudio software is employed to assess the impact of seismic forces on stability and suggest necessary solutions. The results of the analysis revealed that the factor of safety (FOS) at all three locations was critically low, i.e., falling below the value of 1, which indicates a heightened risk of slope instability. In response to these findings, solutions are proposed for each location to enhance stability and mitigate the risk of failure. For Chainage 9 + 110, the solutions involve the implementation of gabion at berms and retaining wall at the toe to provide additional lateral support. For Chainage 10 + 580, where the stability was particularly precarious a combination of anchors as well as gabion along with a retaining wall at the toe is provided. Chainage 11 + 300 KM showed stable condition before and after application of seismic load, hence no remedial measure is implemented. The suggested solutions highlight the significance of site investigations and software application in addressing the particular challenges posed by seismic activity. In the following study focus is given to address the identified risks, ensuring the long-term safety and integrity of the railway slope.