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Integrated pest management (IPM) techniques employing insect-fungal interactions could utilize “entomopathogenic fungi (EPF)” to control insect pests. This approach has garnered significant attention; however, it has seen limited application in combating the brown planthopper, Nilaparvata lugens, a serious rice pest in Thailand and other countries. The goals of this research were to isolate Cordyceps javanica PSUC001 based on morphological and molecular genetic traits and to evaluate its activity against N. lugens using histological methods. The fungi were isolated from infected moth on Khohong Mountain, Songkhla, Thailand. Live adult specimens were inoculated with the isolated C. javanica and examined morpho-histologically from 0 to 108 h post-inoculation. Molecular genetics analysis computed the identity of C. javanica PSUC001, and the initial degeneration of spermatozoa of N. lugens at 48 h post-inoculation. The necrotic spermatozoa of the sampled insects were observed within 108 h among the prominent fungi formation. After thorough evaluation, our research demonstrated a correlation between C. javanica PSUC001 activity and N. lugens, which could induce spermatozoa histopathological changes to understand the fungal destruction and host physiological reactions, aiming to increase mortality and provide effective pest management solutions.

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Molecular Genetic Identification of Cordyceps Javanica PSUC001 and Its Potential Control Strategy of Nilaparvata Lugens (Stål)

  • 会议论文

  • 发表日期：

    2025年

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  • Peerasak Bunsap,
  • Sinlapachai Senarat,
  • Seree Niyomdecha,
  • Chaninun Pornsuriya,
  • Narit Thaochan

摘要

Integrated pest management (IPM) techniques employing insect-fungal interactions could utilize “entomopathogenic fungi (EPF)” to control insect pests. This approach has garnered significant attention; however, it has seen limited application in combating the brown planthopper, Nilaparvata lugens, a serious rice pest in Thailand and other countries. The goals of this research were to isolate Cordyceps javanica PSUC001 based on morphological and molecular genetic traits and to evaluate its activity against N. lugens using histological methods. The fungi were isolated from infected moth on Khohong Mountain, Songkhla, Thailand. Live adult specimens were inoculated with the isolated C. javanica and examined morpho-histologically from 0 to 108 h post-inoculation. Molecular genetics analysis computed the identity of C. javanica PSUC001, and the initial degeneration of spermatozoa of N. lugens at 48 h post-inoculation. The necrotic spermatozoa of the sampled insects were observed within 108 h among the prominent fungi formation. After thorough evaluation, our research demonstrated a correlation between C. javanica PSUC001 activity and N. lugens, which could induce spermatozoa histopathological changes to understand the fungal destruction and host physiological reactions, aiming to increase mortality and provide effective pest management solutions.