Galloping of transmission lines is extremely destructive and threatening to the safe and stable operation of lines, which can lead to windage flashover of insulators, flashover between lines, damage of fittings and so on. Therefore, it is necessary to study and analyze the anti-galloping of transmission lines. In this paper, the anti-galloping effect of interphase spacers is verified by comparative analysis of multiple arrangement and number distribution of interphase spacers. The interphase spacers were installed on the tower-line system model, and the effectiveness of spacer anti-galloping was analyzed according to different arrangement modes and different numbers of spacers. The results show that the anti-galloping effect is the best when the transmission line is equipped with the special arrangement of three spacers at a span of 200m in random wind field of 15m/s. The vertical amplitude of the transmission line decreased by 56.08%, and the lateral amplitude decreased by 37.96%. It shows that the interphase spacer applied to the galloping area of 110 kV Liannan transmission line has a certain anti-galloping effect.