Annually, a considerable quantity of rice husks is discarded post-harvest due to inadequate utilization. Nevertheless, this valuable agricultural byproduct harbors an opportunity for the development of high-value-added, high-performance, and multifunctional bio-inspired nanocomposites. These applications span various sectors, including energy storage, food industries, hygienic products, and cosmetics. This study explores a facile one-pot process for extracting biogenic silica from rice husks, aiming to produce high-purity and homogenous silica nanoparticles. Rice husk samples of Oryza Sativa var. Indica was randomly collected. Following drying and grinding, the samples underwent acid leaching with sulfuric and hydrochloric acids. Subsequently, both acid-leached and un-leached samples were then subjected to calcination at temperatures of 500, 600, 700, 800, and 900 °C. The silica content was quantitatively assessed using sophisticated equipment, including X-ray fluorescence (XRF) and Field Emission Scanning Electron Microscopy (FESEM). XRF analysis validated these findings, demonstrating that the silica purity in acid-leached samples surpassed that in non-acid-leached samples, with purity exceeding 96% in sulfuric acid-leached samples. FESEM analysis further corroborated these results, revealing an average spherical biogenic nano-silica size of less than 12 hydrochloric acid-leached samples and 10 nm for sulfuric acid-leached rice husks.