Scholarlink

This chapter explores innovative methodologies for studying B cell activation and B cell receptor (BCR) signaling. It emphasizes the use of surrogate antigens tethered to lipid bilayer membranes and varying stiffness matrices, along with photoactive antigen presentation systems. Advanced techniques such as traction force microscopy and site-specific labeling of BCR and soluble immunoglobulin are discussed to measure B cell mechanical sensing and BCR conformational changes. The chapter additionally details an alternative method for tethering IgG surrogate antigens using the H12-D-domain on Ni2+-containing lipid bilayers, which facilitates live cell and single-molecule imaging experiments. Additionally, it investigates the effect of substrate stiffness on B cell activation, proliferation, and antibody responses, revealing that B cells are sensitive to antigen-presenting substrate rigidity. The chapter also introduces a photoactivatable antigen presentation system to precisely control and analyze early B cell activation events. These methodologies provide a deeper understanding of B cell function and regulation, offering insights into potential immunotherapeutic targets.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Techniques for Antigen-Induced B Cell Activation Imaging

  • 章节

  • 发表日期：

    2025年

查看全文
  • Yingyue Zeng,
  • Yue Xu,
  • Yuxin Li,
  • Hao Yang

摘要

This chapter explores innovative methodologies for studying B cell activation and B cell receptor (BCR) signaling. It emphasizes the use of surrogate antigens tethered to lipid bilayer membranes and varying stiffness matrices, along with photoactive antigen presentation systems. Advanced techniques such as traction force microscopy and site-specific labeling of BCR and soluble immunoglobulin are discussed to measure B cell mechanical sensing and BCR conformational changes. The chapter additionally details an alternative method for tethering IgG surrogate antigens using the H12-D-domain on Ni2+-containing lipid bilayers, which facilitates live cell and single-molecule imaging experiments. Additionally, it investigates the effect of substrate stiffness on B cell activation, proliferation, and antibody responses, revealing that B cells are sensitive to antigen-presenting substrate rigidity. The chapter also introduces a photoactivatable antigen presentation system to precisely control and analyze early B cell activation events. These methodologies provide a deeper understanding of B cell function and regulation, offering insights into potential immunotherapeutic targets.