The evolving landscape of aquaculture has necessitated advancements in the management of antibiotics to enhance efficacy, ensure biosafety, and minimize environmental impact. Recent research in pharmacokinetics has provided deeper insights into the absorption, distribution, metabolism, and excretion of antibiotics in aquatic species, leading to improved dose regimens and reduced resistance development. Advances in efficacy assessments have refined strategies for optimizing therapeutic outcomes while managing microbial resistance. Concurrently, efforts to enhance biosafety have focused on mitigating the risks associated with antibiotic residues, safeguarding both human health and aquatic ecosystems. The development of novel withdrawal protocols has been crucial in ensuring that antibiotics are effectively cleared from aquatic species before they enter the food chain, thus preventing potential adverse effects.