Biofuel regulatory frameworks at both national and international levels aim to promote sustainable fuel alternatives, reduce greenhouse gas emissions, and ensure energy security. These frameworks also govern the production, distribution, and sustainability standards of biofuels. These collectively support the transition to cleaner energy while addressing food security, land use, and economic feasibility. Most countries, including India, have their own set of regulations, directives, and policies to regulate the use of biofuels. Biofuel regulatory frameworks are essential at both national and international levels to guide the sustainable growth of the biofuel sector. These regulatory charters establish standards to prevent environmental degradation, reduce greenhouse gas emissions, and promote the use of domestic renewable resources, thereby reducing dependence on fossil fuel imports and enhancing energy resilience. They monitor land use for biofuels, taking into account food security, and aim to attract investment, support innovation, and create a stable environment for industry growth. As different nations move toward adopting biofuels, these regulations and international standards help align sustainability criteria across countries, enabling fair trade and cooperation in biofuel development. These countries recognize the use of lignocellulosic biomass for generating biofuels, and regulatory frameworks are evolving globally to support their development due to their low carbon footprint and minimal impact on food security. The regulations implemented worldwide are crucial to ensuring compliance and maintaining a balance between food and fuel production. These frameworks ensure that biofuels are a clean, responsible, and viable part of the global energy future.