Confirmatory detection of blood evidence holds a huge significance in the criminal justice system. Samples can be collected from the crime scene after conducting the presumptive examination. However, either field based or laboratory-based tests need to be performed before using the samples for source attribution by forensic DNA examination. The primitive examinations such as the crystal tests have been replaced with more advanced immunochromatographic techniques targeting the human-specific antigens present on blood cells. Such tests not only provide confirmatory test results for blood, but also human origin of the blood samples. Besides, immunochromatographic techniques are highly robust and can be used for on filed examinations. mRNA and miRNA-based tests are also emerging. However, such tests need more standardization before their application in the case work samples.