Technological advancements in imaging have significantly enhanced our understanding of microbial systems, transitioning us from crude, reversed telescopic designs to advanced platforms that can visualize intracellular activities in real-time. This development has been accelerated further with the use of computational tools and artificial intelligence (AI), which have significantly reduced human error and enhanced precision, while facilitating automated analysis, high-throughput data collection, and efficient storage. Contemporary imaging techniques have overcome many of the drawbacks of traditional optical microscopy, providing unmatched spatial and temporal resolution. This chapter focuses on significant developments that have made distinct contributions to microbial imaging, including fluorescence microscopy, cryo-electron microscopy, and atomic force microscopy (AFM). AFM offers three-dimensional topographical and mechanical data at the nanoscale. Cryo-EM enables near-atomic-scale resolution imaging of cellular and molecular structures, while fluorescence microscopy enables targeted imaging of biomolecules through specific labelling. Together, these approaches have enhanced our understanding of the morphology, physiology, and dynamic behavior of microorganisms. The chapter also examines the expanding field of multi-modal and correlative imaging, which combines several imaging modalities to produce complementary data. By utilizing the advantages of each modality, this convergence enables researchers to tackle a variety of imaging difficulties, including contrast restrictions, resolution gaps, and sample preservation. Thus, a paradigm change in microbial research is represented by the cooperative application of sophisticated imaging and computational integration, which provides a comprehensive and multifaceted picture of microbial life that connects structural and functional discoveries at the molecular and cellular levels.