For centuries, people have been interested in natural dyes for dyeing applications. Natural dyes are known for their phytochemical properties, which are highly sought-after in many fields, including dyeing. The extraction of compounds from plant matter is the key to obtaining the dyestuff. A number of research projects have focused on finding and developing better extraction methods. Modern environmentally-friendly extraction methods, such as microwaves, accelerated solvents and ultrasound, are in the ascendancy. The aim of this study was to compare three methods of extracting natural dyes from the bark of Mitragyna Inermis, a plant belonging to the Rubiaceae family, for use in textiles. The methods evaluated were maceration, digestion and ultrasound- assisted extraction. The three extraction methods were optimized according to the parameter’s extraction temperature and extraction time. The effectiveness of each method was assessed by calculating the extraction yield after optimization and measuring the colour strength (K/S) after application to fabric.