The development of a glycan sequencer remains a demanding task, as glycans cannot be amplified like DNA nor can they be identified from partial structures using tandem mass spectrometry (MS/MS) in the same manner as proteins. On the other hand, the automation of glycan profiling has already been partially realized (Fig. 4.1) Solid technologies such as liquid handling robots, microfluidics, and magnetic beads are employed in sample preparation, often in combination with resins for purification. After derivatization, glycans are separated—if necessary—using liquid chromatography (e.g., high-performance liquid chromatography (HPLC)) or multi-capillary electrophoresis and then detected by mass spectrometry or fluorescence spectrometry [1, 2].