The structural analysis of glycans, glycopeptides, and glycolipids has become possible by utilizing liquid chromatography-mass spectrometry (LC-MS) analysis and biochemical methods. Specific targets such as nucleotide sugars have also been quantified using triple quadrupole MS [1]. Furthermore, improvements in LC and the development of column particle technologies are continuously being pursued in order to enhance the quantitative reproducibility. Moreover, comprehensive analysis using Orbitrap MS is accelerating research that delves into the limits of each omics analysis [2]. Ion mobility technology further enhances the comprehensiveness of omics by separating sugar isomers. A new fragmentation approach, called electron-activated dissociation (EAD), is also being considered for the analysis of O-type glycopeptides [3] (Fig. 2.1).