Caenorhabditis elegans, a transparent soil-dwelling nematode, is widely studied as a model organism in different research fields. Its short life cycle, simple body design, and about 60–80% genetic similarity to humans make it useful for studying developmental biology, neurobiology, aging, and wound healing. These are maintained on high growth medium (HGM) plates seeded with Escherichia coli strain (OP50), which serves as a food source. The process of routine culture involves preparing nutrient agar plates, transferring worms through chunking, and storing the plates at an optimal temperature to ensure the proper development of C. elegans from embryo to adult. Moreover, to obtain an age-synchronized culture, homogeneous culturing techniques are employed. This involves bleaching of the culture, which removes all the larval as well as the adult worms, leaving only embryos that hatch and develop simultaneously. Age synchronization helps to study life span and stage-specific gene expression. Sub-lethal UV exposure of C. elegans causes tissue damage at a specific location without eradicating the entire organism. The cell or tissue rupture can be seen by using dual acridine orange–ethidium bromide (AO–EtBr) staining. This localized damage helps to study the repair mechanisms and stress responses of the organism. Since C. elegans shares significant genetic similarity with humans, such studies provide insights into the cellular processes underlying DNA damage, apoptosis, and recovery. Furthermore, the transparency of the worm makes it an excellent model to visually track morphological changes and evaluate the extent of UV-induced injury.