The small and large intestines possess inherent motility due to the coordinated actions of interstitial cells of Cajal, the myenteric plexus, and smooth muscle. Normal small intestinal motility exhibits distinct patterns during fasting and postprandial periods. Fasting motility consists of phase I (quiescence), phase II (sporadic irregular contractions), and phase III (regular strong contractions at a frequency of 3 cycles per minute, propelling contents anally). This sequence is known as the migrating motor complex. Normal colonic motility comprises three components: segmentation contractions, propulsive movements, and high-amplitude propagating contractions. Physical stimulation of the intestinal lumen triggers reflexive movements (peristaltic reflex, intestino-intestinal inhibitory reflex) and visceral perception. Several methods exist for measuring intestinal motility. In this chapter, we focus on intraluminal pressure measurement and barostat techniques for assessing gastrointestinal wall movements. Intraluminal pressure measurement and barostat remain the most effective methods for the detailed evaluation of motility patterns from the upper to lower gastrointestinal tract.