Cellular communication in bioelectromagnetics relies on electromagnetic signal transduction, critically influencing physiological processes. Optical tweezers, utilizing photon momentum transfer for non-contact trapping forces, are a powerful tool for manipulating micro/nano-scale biological entities and probing bioelectromagnetic interactions. However, precise control of particle dynamics is highly dependent on laser parameters, with the quantitative relationship between laser power and motion characteristics remaining inadequately explored, particularly in three-dimensional aqueous environments. This study systematically investigates the effect of laser power (56–96 mW) on the dynamics of 6-μm polystyrene particles in aqueous solution using optical tweezers. Results demonstrate that particle motion within this power range is dominated by isotropic Brownian dynamics. Quantitative trajectory analysis revealed a weak but observable reduction in the particle displacement fluctuation range as laser power increased, evidenced by a 7.7% decrease in mean motion diameter (from 13.0 × 10−7 m at 56 mW to 12.0 × 10−7 m at 96 mW). Crucially, an operational upper power limit of 150 mW was identified, beyond which significant optical trap positioning deviations led to particle capture failure. Environmental mechanical vibrations were also found to compromise trapping stability and tracking accuracy. These findings quantify the power-dynamic response in optical trapping and provide essential parameter optimization guidelines—specifically, maintaining laser power below 150 mW and implementing enhanced vibration isolation—to improve the precision and reliability of optical manipulation for bioelectromagnetic research and non-contact cell/organelle handling applications.