Frataxin is a highly conserved and important mitochondrial protein that plays a key role in iron metabolism and regulation of oxidative stress, and is involved in maintaining intracellular iron homeostasis and attenuating reactive oxygen species (ROS) accumulation. Hypomagnetic fields interfere with animal behavior and cellular function by affecting the level of oxidative stress. Previous laboratory studies have shown that magnetic field modulation can affect frataxin expression. Therefore, this study explored the potential mechanism of action on frataxin function and oxidative stress through hypomagnetic field conditions. The results showed that the hypomagnetic field had multiple effects on FXN-deficient Drosophila, while delaying the developmental calendar period and improving offspring fecundity.