COVID-19 pandemic underscored the importance of point of care (PoC) to improve the detection and treatment in minimal resources and manpower as numerous challenges arose during crisis. Additionally, pandemic also emphasizes the need for personalized medicine. Personalized medicine represents a shift from the traditional “one-size-fits-all” approach to a more customized, individualized treatment strategy that considers the unique genetic, environmental, and lifestyle factors of each patient. The ultimate goal of personalized medicine is to treat patients at the earliest to prevent adverse events that matter to both the clinician and patients. The emergence of 3D printing in healthcare has triggered a revolution in the development of PoC devices by eliminating the reliance on centralized facilities; these devices allow for effective medical interventions even in low-resource settings. Their portability, ease of use, and ability to provide rapid, accurate results make them essential in both emergency and routine healthcare scenarios. This chapter outlines the principle of 3D printing, steps involved, healthcare applications in the development of PoC devices, and its benefits to patients—from concept to real clinical applications, i.e., microfluidic devices, wearable biosensors, and lab-on-a-chip systems, with case studies in infectious disease diagnostics. We also explored regulatory challenges, material standardization, and current technological limits. Future advances in smart materials and bio printing are poised to elevate PoC capabilities, making 3D printing a key driver of faster, more precise, and accessible personalized healthcare.