The properties of biofuel produced by pyrolyzing feedstock made from M. indica seed cake and P. dulce fruit coat, which were chosen due to their high volatile matter content. To characterize the pyrolysis oil, char, and gas that resulted, a fast pyrolysis procedure was carried out in a fixed-bed reactor at between 350 and 600 °C temperature range. The greatest pyrolytic oil output of 44.2 wt%, along with char and gas yields of 22.1 and 33.7 wt%, respectively, was obtained at 475 °C, after the impact of process temperature on product distribution was examined. The viability of M. indica seed cake and P. dulce fruit coat as sustainable energy sources was confirmed by thermogravimetric analysis (TGA) and Fourier transform infrared spectroscopy (FT-IR), which were used to determine feedstock suitability. Modern analytical methods like 1H NMR, FT-IR, and GC–MS were used to characterize the pyrolysis products. In contrast to regular diesel, the pyrolysis oil has a lower heating value and is a viscous liquid, according to physical and chemical research. Thorough 1H NMR, FT-IR and GC–MS analyses revealed high concentrations of oxygenated substances, such as acidic compounds and phenol derivatives. The bio-oil’s FT-IR analysis verified the existence of aliphatic and aromatic hydrocarbons, while gas chromatography of the pyrolytic bio-oil showed the presence of acidic, oxygen containing, and combustible compounds.