Telemedicine has emerged as a transformative approach to delivering healthcare services, particularly during the COVID-19 pandemic, by enabling remote access to medical consultations and monitoring. However, many healthcare workers remain inadequately prepared for this digital shift. This study aimed to evaluate healthcare workers’ readiness for telemedicine implementation across four key dimensions: core readiness, e-learning readiness, clinical readiness, and technology readiness. A cross-sectional descriptive study was conducted using convenience sampling. Data were collected through an online questionnaire adapted from the Core, Clinical, and E-Learning Readiness Assessment and the Technology Readiness Assessment tools. A total of 60 healthcare workers participated voluntarily. Descriptive statistics were used to analyze readiness levels in each domain. Among respondents, 30% demonstrated readiness in the core readiness domain, 95% showed high readiness in e-learning, 63% in clinical readiness, and 68% in technology readiness. These findings indicate that while healthcare workers are generally prepared in e-learning, clinical, and technological aspects, fundamental readiness concerning motivation, commitment, and institutional support remains limited. Healthcare workers exhibit strong readiness in adopting digital-based healthcare services, particularly in technological, clinical, and educational aspects. However, low core readiness underscores the need for organizational engagement, policy reinforcement, and attitudinal change before large-scale telemedicine adoption can be achieved.