The study focuses on the process of generating biofuel from rural waste that is made from sugarcane bagasse by pyrolysis. The objective is to enhance pyrolysis to generate advanced biofuels and enhance sustainable energy production for its global demand. An overview of biofuels includes characterization and significant effect in the field. The report also outlines the setup procedure, experimental setup, characteristics of materials, and worldwide production trends. Temperature, particle size, bed height, heating rate, and N2 flow rate are adjusted to enhance pyrolysis efficiency. The study conducted three case studies focusing on certain conditions for a higher production of bio-oil yield. Therefore, the highest pyrolysis conditions are temperature 500 °C, particle size 0.3 m, bed height 16 cm, bio-oil 44.23 wt%. In the second case temperature 550 °C, 500 mm particle size, heating rate of 20 °C/min, with produced 43.32 wt%. Furthermore, in the third case study, at 500 °C, the bio-oil yield boosted to 45 wt% compared to the previous case, with a wide range of particle sizes, and heating rate of 30 °C/min. Also examining bio-oil extraction yields, biochar, and biogas. A maximum amount of bio-oil had been produced in case 2 that was 60.4 wt%, and in case three established the highest biogas and biochar that was 36 wt%. The following study focuses on environmentally friendly procedures and alternative resources that can be considered renewable by examining sugarcane bagasse, for example a source intended for biofuel production process. The outcomes associated with using biofuels instead of conventional energy sources might prevent the emission of greenhouse gas and minimize environmental degradation.