This chapter presents the development of BTC_AFv1, the foundational iteration of the envisioned Bitcoin Audit Framework (BTC_AF), designed to guide auditors in addressing the complexities of auditing Bitcoin-related balances and transactions. Grounded in the object-centric design phase of the elaborated Action Design Research (eADR) methodology, the chapter responds to the book’s objective through a structured framework aligned with International Standards on Auditing (ISA) assertions. Drawing on literature, practitioner insights, and the technical features of the Bitcoin blockchain protocol, the framework outlines detailed procedures that cover key areas, including wallet management, cryptographic ownership verification, and transactional completeness. Each procedure is mapped to relevant assertions, ensuring audit rigor, clarity, and practical relevance. The result is a robust audit tool that integrates theoretical foundations with real-world application, offering clear guidance for auditors engaging with Virtual Asset Service Providers (VASPs) and non-VASPs exposed to the Bitcoin blockchain and its native currency. The BTC_AFv1 thus establishes a foundation for industry validation, positioning itself as a valuable artifact in advancing audit quality and consistency within the evolving digital asset landscape.