Facile structure of the core M3 O-mannosyl glycan has recently been elucidated. The glycan stabilizes muscle tissue by binding to dystroglycan and laminin at the reducing and non-reducing end, respectively. We succeeded to chemically synthesize pivotal parts of the core M3 O-mannosyl glycan including tandem ribitol phosphate and matriglycan. The former acted as primer of the enzymatic glycan elongation. The latter exhibited superior affinity for laminin. Our synthesized glycans may serve as medicine to stabilize the muscle tissue.