The arrival of the epidemic era has led to online teaching activities rapidly sweeping through major universities nationwide. The research about online learning has also been widely spread, but among them, the research about the teacher-student interaction behaviour in online learning is still a little lacking, and the live lecture, as an important teaching form of online learning, is more worthy of exploration and enhancement of its teacher-student interaction behaviour. Therefore, this study takes live lectures as an example, and explores the status quo of teacher-student interaction in online learning among college students through questionnaires, and analyses the results of the survey through SPSS 26.0, and finds that college students are in a passive state in teacher-student interaction of online learning, and lack of interactivity, which this study further explores the factors affecting teacher-student interaction in online learning. Finally, from the perspective of platforms, learners and teachers, we propose enhancement strategies that can effectively optimise teacher-student interaction behaviours.