Tyrosine kinases play key roles in cellular signalling pathways that influence cell proliferation, differentiation and survival. In cancer, these metabolic enzymes are frequently imbalanced because of mutations in genes, amplifications or overexpression, resulting in aberrant cell signalling and tumour growth. Tyrosine kinases interact with metabolic pathways such as glycolysis and non-glycolytic acid metabolism to help tumour cells meet their energy and biosynthetic necessities. This dual role emphasises their significance in connecting oncogenic signalling and reprogramming of metabolism. Exploring the association between tyrosine kinase activity and cancer metabolism could lead to the development of personalised cancer therapies. The interaction with tyrosine kinase signalling and cancer metabolism will allow for new approaches to combat tumour development and metastasis. Future medicines that target these interconnected pathways should more effectively interrupt tumour growth and metastasis while reducing mechanisms of resistance. This chapter discusses the overview of glycolysis, basic concepts and pathways, role of non-glycolytic acid and glycolytic enzymes in cancer progression, tyrosine kinase signalling pathways in cancer, crosstalk between glycolytic enzymes and tyrosine kinase signalling and emerging research and technologies in targeted cancer therapy.