Plant viruses threaten sustainable agriculture and cause large financial losses. The generally utilized procedures cannot completely eradicate viral infection in affected plants. Many novel methods are currently being used to prevent plant viral infection; however, none of these treatments have proven to be effective. The generally utilized procedures cannot completely eradicate the viral load in affected plants. This review focuses on the role of plant growth-promoting rhizobacteria/endophytic bacteria, actinomycetes, and fungal species in preventing plant viral infection. Cross-protection mechanisms, ISR, and the accumulation of defensive enzymes, phenolic compounds, lipopeptides, proteases, and RNases in response to plant virus infection have been addressed.