Water pollution, especially the pollution of surface water resulting from the discharge of industrial and medical waste, has become a significant area of concern in the field of environmental studies. Various methodologies and strategies are utilized for the treatment of these contaminated waters, with the process of adsorption on activated carbon being identified as a promising method for the purification of water. The focus of this research revolves around the exploration of the removal of amoxicillin by employing activated carbon sourced from Prickly Pear Seeds extracted under supercritical carbon dioxide (SC-PPSAC). The response surface methodology was employed to ascertain the optimal conditions for eliminating amoxicillin (AMX) through the utilization of SC-PPSAC. An investigation was conducted on the impact of the experimental factors (initial MB concentration, adsorbent dose, time, and pH) on the adsorption capacity, the optimal parameters included an initial AMX concentration of 140 mg/L, a dosage of 0.1 g of SC-PPSAC, a solution pH of 3, and a time of 120 min. The adsorption kinetics conformed to the model of pseudo-second-order adsorption and demonstrated that the process of adsorption could achieve equilibrium 120-min. The maximum capacity for adsorption of AMX onto our activated carbon calculated to be 17.8 mg/g.