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Nanoparticles are aggregates that range in size from 1 to 100 nm and can be made either naturally or intentionally. They are useful in a variety of domains, including genetic engineering and medicine. Plant cells can be altered by carbon-based nanoparticles, specifically carbon nanodots (CNDs), which provide a biocompatible and economical option for conventional genetic modification (GM) approaches. Arabidopsis thaliana and Triticum aestivum were employed as model species in this study to assess the effectiveness of CNDs in gene delivery. Many transformation techniques, including foliar sprays, vacuum infiltration, and seed treatments, were evaluated. Multiple foliar sprays produced the highest transformation efficiency; seed treatments were ineffective. This chapter explores the increasing significance of carbon-based nanomaterials as non-viral vectors for CRISPR delivery by addressing problems with viral vectors and demonstrating innovative design strategies to optimize interactions between CRISPR components and nanomaterials. The results highlight how carbon-based nanomaterials can improve genetic engineering and gene delivery, opening the door to safer and more effective gene-editing techniques.

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Carbon Nanoparticles in Plant Genetic Engineering

  • 章节

  • 发表日期：

    2026年

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  • Rooma Waqar,
  • Javed Iqbal,
  • Banzeer Ahsan Abbasi,
  • Abdul Samad Mumtaz,
  • Akhtar Munir,
  • Muhammad Kaleem,
  • Muhammad Anas,
  • Muhammad Muzamil Sattar,
  • Asifa Farooqi,
  • Shumaila Ijaz,
  • Zakir Ullah,
  • Ghulam Murtaza,
  • Rashid Iqbal,
  • Sana Basharat,
  • Muhammad Waseem

摘要

Nanoparticles are aggregates that range in size from 1 to 100 nm and can be made either naturally or intentionally. They are useful in a variety of domains, including genetic engineering and medicine. Plant cells can be altered by carbon-based nanoparticles, specifically carbon nanodots (CNDs), which provide a biocompatible and economical option for conventional genetic modification (GM) approaches. Arabidopsis thaliana and Triticum aestivum were employed as model species in this study to assess the effectiveness of CNDs in gene delivery. Many transformation techniques, including foliar sprays, vacuum infiltration, and seed treatments, were evaluated. Multiple foliar sprays produced the highest transformation efficiency; seed treatments were ineffective. This chapter explores the increasing significance of carbon-based nanomaterials as non-viral vectors for CRISPR delivery by addressing problems with viral vectors and demonstrating innovative design strategies to optimize interactions between CRISPR components and nanomaterials. The results highlight how carbon-based nanomaterials can improve genetic engineering and gene delivery, opening the door to safer and more effective gene-editing techniques.