The multifunctionality of acoustic radiation force in manipulating particles enables them to address current challenges in the fields of biomedicine and medicine. The arrangement, separation, and sorting of cells are crucial processes in studying cell characteristics, diagnosing diseases, and treatment, especially for cancer patients. Early detection of cancer cells can greatly aid in cancer treatment. Acoustic radiation force separation of particles offers a promising alternative to traditional techniques, allowing the separation of different particles of varying sizes by adjusting the acoustic radiation force on the particles. In practical applications, it is necessary to transport biological products in a controlled manner.