Concave spherical focusing transducers (Ruo in Ultrasound handbook. Nanjing University Press, 2021; Mingxi et al. in Medical ultrasound—principles and techniques. Xi'an Jiaotong University Press, 2001) have significant applications in the field of biomedical ultrasound, such as the use of high-intensity focused ultrasound (HIFU) for tumor treatment due to its thermal effects (Kennedy in Nat Rev Cancer 5:321–327, 2005), the application of phased array focusing transducers (Wang and Qian in Acoustics letters 10:5–10, 1986), and the cavitation effect of sound waves (Suslick and Price in Annu Rev Mater Res 26:295–326, 1995). This chapter uses an ideal spherical focusing transducer as a model, and by using the ray acoustics approximation, calculates the three-dimensional acoustic radiation force on spherical particles in a high-frequency focused sound field in water. This chapter provides a detailed derivation of the three-dimensional radiation force calculation model, studies the impact of various parameters on the radiation force, and provides the location of the three-dimensional acoustic potential well generated by the spherical focusing sound field on particles. This provides a theoretical basis for the application of spherical focusing transducers in acoustic trapping.