Flexible silicone tubes are highly prized for their superior qualities, such as corrosion resistance, purity, strength, and inertness, making them crucial in medical and industrial applications. This research focuses on the dynamic behavior of twisted silicone tubes under internal excitation from pulsatile flow, employing a validated numerical modeling approach using the finite volume method. The study reveals that during the initial twist from 0 to 150°, the silicone tube exhibits a constant natural frequency. Beyond 150°, the frequency linearly decreases up to 300°, stabilizing thereafter. This exploration provides valuable insights into fluid–structure interactions, enhancing our understanding of silicone tubing dynamics. The findings contribute to optimizing design and performance in medical, marine, and industrial systems utilizing such tubing.