Giant vesicles (GVs) which are closed lipid bilayer membranes with diameters greater than 1 μm have garnered attention for the construction of cell-mimetic molecular robots. When evaluating the operation of functionalized GVs that act as sensors, processors, or actuators, or these complexes, the number of GVs that should be tested increases with the number of components and parts involved. This is due to the variation in the yields of their molecules and modules within the GVs and GV complexes. Consequently, functionalized GVs must be evaluated with high precision within a short timeframe. This necessitates continuous improvements in measurement methods, supported by digital technology and high-performance computing. In this section, we review and discuss emerging digital technologies for evaluating GVs.