De novo-designed artificial ion channels, including DNA, peptide, and organic compound, attract attentions as the sensor of molecular robots because they can emerge various stimulus responsiveness. To apply artificial ion channels as the sensor of molecular robots, it is necessary to understand the detailed characteristics of ion channels, such as channel structure, stability in the artificial cell membranes, molecular transportation, and molecular selectivity. Although the ion current measurement is one of the powerful methods to evaluate the characteristics of artificial ion channels, the low throughput of ion current measurements using conventional methods to form artificial cell membranes prevents the efficient evaluation of artificial ion channels. In this chapter, we introduce probe-type artificial cell membrane systems that can improve the throughput of ion current measurements.